check
Success: Your bookmark has been successfully created.
check
Success: Your bookmark has been successfully removed.
check
Success: Password updated and now signed in
error_outline
Success: Your password has been updated

Available to following accounts
Lite
Academic
Premier
Sponsored by
HPLC Analysis of Oligonucleotides

In this session we present an overview of the HPLC analysis of oligonucleotides. We’ll provide the basics on nucleotides and how synthetic oligonucleotides are produced, introducing you to some of the language used in support of these molecules. We’ll then take a good look at both anion exchange and ion-paired reversed phase analysis, moving on to an introduction to the application of mass spectrometry to oligonucleotides.

 

Topics include:

  • Biotechnology
  • Oligonucleotides overview
  • Therapeutic oligonucleotides
  • Oligonucleotide synthesis
  • Oligonucleotide chromatographic considerations
    • Anion exchange chromatography
    • Ion pair reversed phase chromatography
  • Mass spectrometry considerations
    • Ion pair-MS
    • Intact mass spectrum
    • IP-MS/MS

Learn more with related CHROMacademy content: