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Size exclusion chromatography (SEC) is considered a reference method for the qualitative and quantitative analysis of aggregates of proteins such as monoclonal antibodies (mAbs) and antibody drug conjugates (ADCs). Superior performance in high resolution, high efficiency, and high throughput analysis of mAbs, ADCs and fusion proteins can be achieved with Agilent’s new SEC column, with optimized sub-2 µm particle and unique hydrophilic bonding chemistry.


Topics include:

  • High resolution, high throughput aggregate and fragment analysis using a sub-2 µm SEC column
  • Biologics characterization using high efficiency size exclusion chromatography
  • Integrated SEC-MS analysis of monoclonal antibody (mAb) and antibody drug conjugates (ADCs) under native conditions
  • Tips and tricks for aggregate characterization method optimization