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HPLC Analysis of Oligonucleotides

In this session we present an overview of the HPLC analysis of oligonucleotides. We’ll provide the basics on nucleotides and how synthetic oligonucleotides are produced, introducing you to some of the language used in support of these molecules. We’ll then take a good look at both anion exchange and ion-paired reversed phase analysis, moving on to an introduction to the application of mass spectrometry to oligonucleotides.


Topics include:

  • Biotechnology
  • Oligonucleotides overview
  • Therapeutic oligonucleotides
  • Oligonucleotide synthesis
  • Oligonucleotide chromatographic considerations
    • Anion exchange chromatography
    • Ion pair reversed phase chromatography
  • Mass spectrometry considerations
    • Ion pair-MS
    • Intact mass spectrum
    • IP-MS/MS

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