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Internal standards are an approach we use to improve method precision. Although our aim during method development is to always achieve methods with the best precision we can, there are certain applications that fundamentally cannot deliver good precision. This module will consider all of the practicalities of selecting and using internal standards correctly.


Topics include:

  • How do internal standards work?
  • When not to use internal standards
  • Choosing an internal standard
  • Selecting the concentration
  • Evaluating an internal standard
  • Isotopically labelled standards for mass spectrometry