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The aim of this module is to explain the principles of ion pair chromatography (IPHPLC), and to demonstrate the circumstances under which this approach might be used. We investigate the common reagents used for IPHPLC and explain how to choose the appropriate reagents. We also demonstrate the effects of the important parameters associated with IPHPLC, concentrating on ion reagent choice and concentration. 

At the end of this module you will be able to illustrate how to practically optimise IPHPLC separations as well as demonstrate applications of IPHPLC, including the use of volatile reagents for LC-MS.


Topics include:

  • Fundamental Mechanisms of ion pair chromatography
  • Ion pair reagents
  • Retention & selectivity
  • Important parameters in ion pair chromatography
  • Optimising ion pair concentration
  • Applications & ion pairing for LC-MS
  • Practical ion pair chromatography
  • Getting started with ion pair HPLC