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Flow rates used in conventional LC separations using non-mass spectrometric detectors may not be compatible with LC-MS systems depending upon the eluent system and the atmospheric pressure ionization (API) interface type used.

 

Topics include:

  • Flow limitations of various LC-MS API sources and solutions for use with conventional HPLC systems
  • Application and benefits of using micro and nano flow in LC-MS
  • Pre and post column flow splitting and quantitative nature of flow splitting