Primary Sample Preparation Techniques

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Primary Sample Preparation Techniques

This module takes an initial look at the science behind the most commonly used sample preparation techniques.

By the end of this unit, you should be able to explain the concepts of protein precipitation, choose appropriate extraction approaches for various analyte and sample matrix types, decide when to use centrifugation (or ultracentrifugation) and understand what can be achieved, identify situations in which simple sample preparation techniques (dilution, filtration, centrifugation etc.) are sufficient to overcome practical problems.

Topics include:

Primary sample preparation techniques
Sample dilution
Sample filtration and ultrafiltration
Centrifugation and ultracentrifugation
Protein precipitation
Extraction approaches to sample preparation
Selectivity
The partition coefficient
Liquid/liquid extraction
Support-assisted liquid/liquid extraction
Solid phase extraction

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