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A UV/visible spectrophotometer is a device used to measure the transmittance or absorbance of a sample as a function of the wavelength of the electromagnetic spectrum. This module covers the fundamental scientific principles behind UV-visible spectroscopy, the principles of the instrumentation, and practical tips for optimizing the instrument parameters.


Topics include:

  • Bonding theory for UV-visible spectroscopy
  • The electromagnetic spectrum
  • Origin of UV-visible spectra
  • Transmittance and absorbance
  • Selection rules and intensity
  • UV-visible chromophores
  • UV-visible absorption maxima of chromophores
  • UV-visible definitions
  • UV-visible instrumentation
  • Optical arrangements of UV-visible spectrophotometers
  • UV-visible instrument configuration
  • Key UV-visible instrument parameters: spectral resolution and selectivity
  • Accuracy and precision
  • Linear dynamic range, calibration, and drift
  • UV-visible sample handling and measurement
  • Choice of solvent, and effect of solvent, concentration, pH, and temperature on UV-visible spectroscopy
  • Troubleshooting UV-visible spectroscopy