No thanks! I would like to know more about CHROMacademy

 Over 3000 E-Learning topics / 5000 Articles & Applications

Chromatography Troubleshooting Tips

  1. Establish a benchmarking method (and representative data) BEFORE the system develops a fault. This can be either a method you regularly use or a method specifically designed as a system checkout, but it must be 100% reliable. Obtain a comprehensive set of data (e.g. back pressure, repeatability, sensitivity, noise etc.) from this method on the system when it is working properly.

  2. Don’t allow problems to start. Be methodical and meticulous in the way you set up the HPLC and where possible perform preventative maintenance routines..

  3. Clearly define the problem:
    Compare a good chromatogram with the problem one.
    • Retention Times  - do they change for all peaks (including T0) or only some
    • Peak height – are they smaller, larger, show poor repeatability – all peaks/only some
    • Elution order – does this remain identical or does peak order change
    • T0 peak – If it is the same (ie k’ changes) – unlikely to be flowrate related, (as this affects all peaks including RI). If it is just retained peaks affected – could be temperature or gradient related
    • Are there any extra peaks?
    • Has peak shape / width changed

  4. Don’t forget the obvious or assume anything. Check EVERYTHING.

  5. Check the easy things first.

  6. Check one thing at a time.

  7. Keep a written note of every single thing you do. You will quickly either forget or doubt yourself.

  8. Use your benchmarking method to establish if problem is due to either the instrument or the method (i.e. the sample prep, method conditions, column or analyst). i.e. if your benchmarking method gives acceptable results, the problem is not related to an instrument fault..

  9. Only take something to bits as a last resort or if you are reasonably sure this is causing the fault.

  10. Don't forget to use the CHROMacademy troubleshooters :-)

HPLC troubleshooting

GC troubleshooting

View the essential guide archive


Learn more about HPLC Troubleshooting with our Essential Guide Webcasts and Tutorials

HPLC Troubleshooting - Eluents and Solvent Delivery Systems  Webcast Tutorial

HPLC Troubleshooting - Autosampler, Column & Detector Issues  Webcast | Tutorial

Troubleshooting your HPLC Chromatogram Part I – Retention, Efficiency and Peak Shape Issues  Webcast | Tutorial

Troubleshooting your HPLC Chromatogram Part II - Selectivity, Resolution and Baseline Issues  Webcast Tutorial


group  subsCHROMacademy can deliver to corporate clients on a multi-user subscription basis.
Served up from secure servers to the corporate intranet or individual desktops.

  • Microsite - your own learning site powered by CHROMacademy
  • Your Landing Pages -with your logo and branding
  • Customized Assessments - Based on content agreed upon Certificate of Completion
  • Certification Programs - Offer your learners a goal to strive towards
  • LMS : Connect - Our Learning Management System is S.C.O.R.M. compliant and will connect to your system
  • Engagement Package - E-newsletter stimulation program derived from your content and ours
  • Full archive of Essential Guide webcasts & tutorials
  • 1000’s of eLearning topics - HPLC / GC / Sample Prep / Mass Spec
  • Ask the Expert - our experts will answer your chromatography questions within 24 hrs.
  • Assessments - test your knowledge
  • Application notes & LCGC articles
  • Troubleshooting and virtual lab tools

Request a quote


 Home | About UsContact Us | SubscribeTerms and Conditions | Advertise | Privacy Policy 

loading data

loading data

loading data


loading data

loading data