In session 1 we will begin the course with a general overview of LC-MS as an analysis technique, what the benefits are and what sort of samples the technique is best suited to.
We will then move on to a more in-depth look at the HPLC section of this hyphenated technique – concentrating on common parts that make up a typical HPLC system and how they work together to deliver successful chromatographic separations.
The session will conclude with a comprehensive overview of the three predominant mechanisms for generating gas phase ions using atmospheric pressure ionization; atmospheric pressure chemical ionization (APCI), atmospheric pressure photo ionization (APPI), and electrospray ionization (ESI). We will discuss the similarities and differences between the methods, as well as which is best suited to the different sample types.
Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.
Tony Taylor B.Sc. CChem FRSC Cert. Ed.
Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.