Ion Exchange Techniques

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Ion Exchange Techniques

Ion exchange chromatography (IEX) is based on the different affinities of analyte ions for oppositely charged ionic functional groups (ligands) bound to the stationary phase surface, and the ability of these analyte ions to displace counter ions associated with the stationary phase ligands. This module discusses ion exchange techniques and applications for the analysis of biomolecules.

Topics include:

Ion exchange chromatography (IEX)
Ion exchange mechanisms
Ion exchange elution considerations
Ion exchange chromatographic process
Ion exchange chromatography for the analysis of biomolecules
Salt gradient elution conditions
pH gradient elution conditions
Column selection for biomolecule analysis by IEX
Anion exchange chromatography for biomolecules
Ion exchange chromatography and MS detection
Ion exchange method development and initial conditions

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