Mass Analyzers Used in Biomolecule Analysis

The working principles of time-of-flight (ToF), orbitrap, and fourier transform ion cyclotron resonance (FT-ICR) mass analyzer will be discussed in this module.

Topics include:

• Time-of-flight (ToF) mass analyzers
• Time-of-flight (Tof) resolution
• ToF - the reflectron
• ToF - increased resolution using reflectrons
• ToF - performance limitations
• Orthogonally accelerated ToF instruments
• oaToF pusher electrode
• oaToF pusher pulse rate
• oaToF deflecting voltage
• oaToF interfacing details
• oaToF mass resolution and accuracy
• oaToF dynamic range
• Orbitrap overview and anatomy
• Orbitrap - continuous ion source
• Orbitrap - ion injection
• Orbitrap - Fourier transform mode
• Orbitrap - mass selective instability (MSI) mode
• Orbitrap - multiply charged ions and calibration
• Orbitrap - high molecular weight application
• Orbitrap - low molecular weight application
• Orbitrap - comparison with other analyzers
• Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)