Method Development for Ion Exchange Using Dynamic Buffer Generation

This webcast will focus on understanding the complexity of biotherapeutic protein charge variant analysis and how optimization of weak cation exchange chromatography is a necessity. Development of robust experimental method conditions can only be found by a systematic screening approach, since each protein will retained differently depending on its sequence and quaternary structure. Coupling the optimized method to a mass spectrometry detector can be achieved by using offline or online (2D) desalting techniques to enable each peak to be fully characterized.

Topics include:

• Charge variants and how they arise
• Use of cation exchange chromatography to analyze native protein charge variants
• Use of software to dynamically create changing buffer conditions for method optimization
• Transferring to MS-compatible mobile phase for greater understanding of individual charge variants