Intact and Subunit LC-MS Characterization of mAb and msAb using Advanced Protein A Columns

Monday, December 8, 2025
8am PST | 11am EST | 4pm GMT | 5pm CET

Obtaining MS-based intact and subunit characterization of monoclonal antibodies (mAbs) and multispecifics (msAbs) is a crucial step in the development workflow. However, older analytical methods often fall short, lacking the depth of information required to support regulatory approval for today’s advanced therapeutics. This webinar introduces an innovative Protein A-MS pH-elution gradient method for achieving effective separations of protein variants with modulated binding affinities prior to direct intact high-resolution MS analysis. We will discuss how advanced ProA-MS methods can overcome traditional limitations and deliver the characterization needed to meet the evolving demands of advancing protein therapeutics.  

 

Topics include:

  • How to achieve in-depth characterization of mAbs and msAbs without prior sample preparation
  • How new Protein A Affinity columns can provide valuable information beyond just titer
  • How Protein A Affinity columns can be used in methods to offer additional characterization insights
  • How to implement unique LC-MS analytical methods for both mAbs and msAbs

 

Speakers

Dr. Stephan Koza (Consulting Scientist, Waters Corporation)
Arnaud Delobel (Director, R&D and Innovation, Quality Assistance).

You are already a member of CHROMacademy.
Please sign in to pre-populate the form and register for the webcast.

You are already registered for this webcast.
Please sign in to enter the webcast lobby.

Complete this form to register for the webcast and receive free Lite membership to CHROMacademy

Already registered?

(* indicates required field)

If you are having trouble registering for or viewing this webcast, please contact us

Terms and conditions

By registering for this event, you agree to MJH Life Sciences® Terms and Conditions, Privacy Policy, and understand your data will be used by MJH and its affiliates for amongst other things, alerting you to other services or products that you may be interested in, and may be shared with or sold to third parties for their use, including possible contact with you through email or other communications, consistent with their respective privacy policy. If you do not agree to have your data used in this way, please contact [email protected]. By receiving FREE Lite membership to CHROMacademy, you agree to the CHROMacademy Terms and Conditions and Privacy Policy, as well as confirming you are OK with CHROMacademy emailing you about your membership and FREE to access content; including the CHROMacademy newsletter, webcasts, and promotions.