Reversed Phase - Introduction and Peptide Level Analysis
Endorsed by
Course Details
Module
Premier
This modules introduces the use of reversed phase HPLC for the characterization of biopharmaceuticals at the peptide level.
Topics include:
- Introduction to reversed phase HPLC
- Analysis at the peptide level
- mAB peptide map
- Common mAb post-translational modifications (PTMs)
- Glycoprofile
- Deamidation
- Oxidation
- Pyroglutamate formation
- Peptide mapping in a routine environment
- Disulfide bridge reduction
- Comparison of digestion enzymes
