Reversed Phase - Peptide Mapping LC Conditions and Effects

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Course Details Module

Author: Crawford Scientific

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This module provides HPLC parameters for carrying out peptide mapping experiments. The effect of each parameter on the resulting chromatography will be detailed.

 

Topics include:

  • Typical peptide mapping conditions
  • Mobile phase additives
  • Temperature
  • Gradient slope/time
  • Column length
  • Particle size and morphology
  • Pore widths
  • Column diameter
Reversed Phase - Peptide Mapping LC Conditions and Effects