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This modules introduces the use of reversed phase HPLC for the characterization of biopharmaceuticals at the peptide level.

 

Topics include:

  • Peptide mapping
  • Common mAb post-translational modifications
  • Glycoprofile
  • Deamidation
  • Oxidation
  • Pyroglutamate formation
  • Disulfide bridge reaction
  • Comparison of digestion enzymes