Reversed Phase - Introduction and Peptide Level Analysis

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Reversed Phase - Introduction and Peptide Level Analysis
Course Details Module Premier
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This modules introduces the use of reversed phase HPLC for the characterization of biopharmaceuticals at the peptide level.

 

Topics include:

  • Introduction to reversed phase HPLC
  • Analysis at the peptide level
  • mAB peptide map
  • Common mAb post-translational modifications (PTMs)
  • Glycoprofile
  • Deamidation
  • Oxidation
  • Pyroglutamate formation
  • Peptide mapping in a routine environment
  • Disulfide bridge reduction
  • Comparison of digestion enzymes
Reversed Phase - Introduction and Peptide Level Analysis