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Where do you start when you want to develop a better HPLC method? Whether you work in a regulated environment or not, setting specifications for your HPLC protocols is a good practice. In this essential guide we will set out guidelines for chromatographic parameters such as retention, resolution, and efficiency that can be used to improve your HPLC methods prior to method validation. We will explain how mobile phase design and instrument parameters can be used to achieve improvements in method robustness and what to look for when a method is failing. Practical tips for sample and eluent preparation, and the correct detector settings to use will also be discussed. Our speakers will look at the major factors that affect chromatographic robustness to give you the tools to develop better methods that will give consistent, reproducible chromatography.

 

Topics Covered:

  • What target specifications should be set for an HPLC method
  • How sample preparation can impact on the sensitivity and robustness of an HPLC method
  • Stationary phase chemistry and important column properties
  • Designing smart eluent solutions
  • Detector parameters to improve quality and robustness
  • Developing inherently robust methods
  • Aspects of LC-MS methods which can (and should!) be optimized