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Although there are good rules to help guide you towards the correct ionization technique, you should always treat each analyte independently and (where practical) perform an infusion of your analyte(s) to select the correct ionization technique and polarity (positive or negative ion mode), and then optimize the critical parameters. This quick guide will provide a step-to-step guide to LC-MS method development and optimization.
This article was written by Dr. Dawn Watson (Technical Expert, Crawford Scientific)