Analytical methods that enable the rapid structural evaluation of monoclonal antibodies mAb, msAb, and fusion proteins are sought after throughout the multiple stages of biopharmaceutical development. One such approach is ProA-MS for the direct analysis of CCCF samples. It has also been demonstrated that the differential dissociation of ProA bound mAb variants can be used analytically when using pH gradient elution. In this study, the impact of using high efficiency affinity columns is evaluated along with extended gradient elution methods for ProA-MS analyses.