Beyond high-sensitivity, high-throughput titer measurements of mAbs, msAbs, and fusion proteins, access to structural characterization data enables more informed development decisions. This application note describes a MS-compatible 4-minute Protein A (ProA)-MS method that combines a high-resolution Xevo™ G3™ QTof Mass Spectrometer with a BioResolve™ Protein A Affinity Column (MaxPeak™ Premier, 3.5 µm, 2.1 × 20 mm) and volatile ammonium acetate/formate buffers. The method enables simultaneous titer determination and detailed glycoform profiling directly from clarified cell culture filtrate (CCCF) samples. Using a NISTmAb spiked into CCCF, the workflow identified most glycoform variants previously reported in a comprehensive denaturing LC-MS study, achieving ~20 ppm mass accuracy. Most variants were detected with just 2 µg of sample, while lower-abundance species required 5-20 µg for confident identification. The results demonstrate that ProA-MS can provide both quantitative titer data and detailed molecular characterization in a rapid, high-throughput format.