CHROMacademy Classroom
HPLC Troubleshooting and Maintenance
Live Online
Instructor-led Training
Resolving a problem with HPLC is usually straightforward. Finding the root cause of the problem within the complex environment of an HPLC analysis is much more challenging. This training seeks to develop a process driven approach to troubleshooting the entire holistic process, allowing delegates to develop skills to be able to tackle any problems.
These skills are applied within a strongly workshop driven environment, where we use many of the most common issues seen with HPLC analysis to consolidate the taught practices.
This is a challenging module and delegates should already have a good understanding of the HPLC process and associated instrumentation.
CHROMacademy Classroom
Delivery Details
Duration: This course is delivered over two Live 4 hour sessions. Run on consecutive days.
Time: Each daily session runs from 9am – 1pm (Eastern Time Zone)
Number of delegates: Maximum of 10.
Price: $810
Course Overview
What You Will Learn:
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How the chromatographic process takes place
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The architecture of a LC system
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Working principles and problems associated with each component of the system
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How to use chromatograms to troubleshoot chromatographic problems
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Best practices for column use and care
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Best practice in system maintenance and performance evaluation
Previous Experience:
Some previous knowledge of HPLC and basic experience are recommended but not essential.
Course content:
The Troubleshooting Process
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Getting the full picture
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Instrument benchmarking
Precisions Issues
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Troubleshooting Contamination Issues
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Extra peaks
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Carryover
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Baseline disturbances
Troubleshooting Column Related Problems
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Physical column damage
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Pressure issues
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Fronting / tailing peaks
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Split peaks / shoulders
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Preventing column issues
Sensitivity Issues
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High noise
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Low signal
Overview of Tackling Method Related Issues
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Tackling Issues Related to Analyst Error
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Common errors during preparation
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Calculation errors