Introduction to Chiral HPLC

Chiral high-performance liquid chromatography (HPLC) is a key analytical technique for the separation and quantification of enantiomers and other stereoisomers. This training module provides a technical introduction to chirality, stereochemical nomenclature, and chiral recognition mechanisms, with a focus on the fundamental chemistry that allows chiral stationary phases to enable enantioselective separations. The course establishes the theoretical and practical foundation required for chiral method development, optimization, and interpretation of chiral HPLC data.

Topics include:

• Back to basics - what is chirality?
• Why do we want chiral purity?
• Structural isomerism
• Stereoisomerism - enantiomers
• Stereoisomerism - diastereoisomers
• Geometric isomers
• E-Z nomenclature
• Epimers and anomers
• Chiral separations