Introduction to SEC-MALS

Learn the fundamentals of size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) and how these techniques are used to characterise the molecular weight, size and structure of macromolecules. Explore the principles of SEC and light scattering, including DLS, RALS, LALS and MALS, the radius of gyration (Rg), the Rayleigh equation and the calculation models used for MALS data analysis. Discover the advantages of combining SEC with MALS for more comprehensive macromolecular characterisation.

Topics include:

• Introduction to size exclusion chromatography (SEC)
• What is light scattering?
• How is light scattering used in GPC/SEC?
• Radius of gyration (Rg) - a size measured by light scattering
• The Raleigh equation
• Dynamic lights scattering (DLS)
• Right angle light scattering (RALS)
• Low angle light scattering (LALS)
• Multi-angle light scattering (MALS)
• LALS and MALS combined
• Comparison of different light scattering technologies
• Different calculation models used in MALS: Zimm model
• Different calculation models used in MALS: Debye model
• Different calculation models used in MALS: Berry model
• Factors that influence LS signals
• Combining SEC with MALS