CHROMtalks - Troubleshooting and Optimizing Sample Introduction in HPLC

While injection in HPLC is now highly controlled, the results are still subject to the same chromatography rules as the original manual injections. Injection solvent, volume, and mass all have an influence on the eventual peak shape as well as other parameters like resolution. This talk will explore the impact of these injection variables on peak shape and provide general recommendations for users who are developing methods. If you already have a method, we will show some simple strategies for improving problems related to the injection process