CHROMacademy Classroom

LC-MS Data Interpretation - Level 1

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Instructor-led Training

This training covers the first two stages in the interpretation of LC-MS data; Picking out compounds of interest in the chromatogram and working with the molecular ion.

This course covers the interpretation of qualitative data generated by a typical LC-MS. We explore techniques thatoptimize the visualization of compounds within a complex chromatogram and work with the molecular ion. The majority of the material can be applied to simple, single-stage mass spectrometers.

We use workshops to build your confidence in working with LC-MS data, highlighting common mistakes and teaching you practical skills that minimize misinterpretation and maximize compound information.

This training is suitable for anyone wishing to develop their understanding and gain confidence in the interpretation of data generated by a typical LC-MS system.

CHROMacademy Classroom

Delivery Details

Duration: This course is delivered over two Live 4 hour sessions. Run on consecutive days.

Time: Each daily session runs from 9am – 1pm (Eastern Time Zone)

Number of delegates: Maximum of 10.

Price: $810 

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Course Overview

What You Will Learn: 

  • Using multiple approaches to visualize compounds of interest within a complex chromatogram 

  • Identification and confirmation of the molecular ion 

  • Extracting additional information from the molecular ion 

Previous Experience: 

Those wishing to attend this course should be experienced in LC-MS methodology and data acquisition.  

Course content: 

Highlighting compounds of interest within the chromatogram 

  • Using collision-induced dissociation effectively 

  • Comparison of CID and MS/MS spectra 

  • Using precursor ion scanning, constant neutral loss scanning and data-dependent experiments in tandem instruments 

  • Improving the quality of data generated in TIC 

  • Use anticipated mass differences (e.g. metabolic transformations) to highlight low concentration compounds of interest 

  • Using contour maps 

  • Using extracted mass effectively 

Identification and confirmation of the molecular ion 

  • Background signals 

  • Multiple charging 

  • Adducts 

  • Influence of fragments of molecular ion confirmation 

Building on the molecular ion 

  • Isotopes

  • Rings and double bond equivalents 

  • Optimizing empirical formula information 

  • Mass delta 

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