Introduction to LC-MS Fragmentation

Thursday, February 19, 2025
8am PST | 11am EST | 4pm GMT | 5pm CET

In this webcast, we break down the relationship between bonding theory and spectral interpretation to explain why fragmentation is a predictable, non-random process. We will discuss the nature of the stable, even-electron ions produced during electrospray ionization and explain how to interpret the structurally unique fragments that result when energy is added to the system.

Throughout this webcast, we will walk through the core mechanisms of charge migration and charge retention, highlighting how specific functional groups and bond energies influence the way a molecule breaks apart. Using practical examples, we will demonstrate how to nominate ionization sites and interpret m/z signals through inductive cleavage and remote hydrogen rearrangements. This webcast offers a clear framework for navigating a complex subject, helping you better understand the logic behind your mass spectrometry data.

Topics include:

• Introduction to LC-MS fragmentation
• Bonding theory
• Overview of electrospray ionization
• Charge migration fragmentation
• Hetero- and homolytic cleavages
• Inductive cleavage
• Charge retention fragmentation
• Remote hydrogen rearrangements

Speaker

Phil Aston (Training and Technical Consultant, Element Lab Solutions)

Colin Towers (Senior Technical Support Chemist, Element Lab Solutions)