Lowering Quantitation Limits for mAb Titer Measurements

Lowering Quantitation Limits for mAb Titer Measurements Using Small Volume 3.5 µm Particle-Size Protein-A Affinity Columns

Protein A (ProA) affinity chromatography (PAAC) is often used for the measurement of monoclonal antibody (mAb) titer in cell culture conditioned media (CM) samples. Here we introduce high-pressure capable and low volume (70 µL) ProA columns that show 5-fold to 7-fold lower LLQ values versus a ProA column constructed with 20 µm porous particles packed into 2.1 x 30 mm PEEK hardware while also delivering comparable performance at higher sample loads. A reduced LLQ can be advantageous when mAb concentrations are low and sample volumes are extremely limited, as when determining titer for milliliter scale micro-bioreactor samples

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