Carryover of monoclonal antibodies or host cell-related impurities is a major pain point in Protein A applications. Release in subsequent elution cycles results in poor reproducibility and introduces variability during titer quantitation, which in turn lowers the achievable limit of quantitation. This application note highlights the observed carryover in Waters BioResolve Protein A columns. The 2.1 x 20 mm format had on average 65% less carryover compared to a leading competitor’s column, and the 3.9 x 5 mm format had no observable carryover. In addition, the application note shows an easy to implement approach to measure carryover in an established method.