This application note highlights a real-world implementation of the BioResolve™ Protein A Affinity Column for routine antibody titer determination in bioprocess development in collaboration with Incyte Corporation. Using a 3-minute method optimized for low carryover, two monoclonal antibodies and one bispecific antibody were analyzed directly from filtered cell culture samples. Compared with a leading third-party Protein A column, the Waters BioResolve Protein A column generated substantially sharper and more symmetrical peaks, reducing peak width by approximately 80% and improving integration, sensitivity, and quantitation reproducibility. The method demonstrated excellent linearity over a 1-10 µg loading range (R² = 0.9996), with a practical limit of quantitation of ~1 µg. Robust performance was maintained across 476 injections using clarified, filtered samples. Based on its superior peak shape, sensitivity, reproducibility, and robustness, the BioResolve Protein A Affinity Column was adopted by Incyte for routine high-throughput antibody titer analysis