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The CHROMacademy Essential Guide Webcast:
N-Glycan Sample Preparation and Analysis Workflows for Screening and Characterization of Biotherapeutics

North America: Wednesday 31st October 2018,
11:00am EDT/ 10:00am CDT/ 8:00am PDT

Europe: Thursday 1st November 2018,
9:00am GMT/ 10:00am CET

Asia Pacific: Friday 2nd November 2018,
8:30am IST/ 11:00am CST/ 12:00pm JST


 

Agilent

This webcast will discuss next-generation sample preparation workflows for enzymatic release and fluorescent dye labeling of N-glycans from biotherapeutic proteins. Benefits and guidance will be given for the selection of fluorescent dyes and choice of analytical separation and detection method (LC/FLR, LC/MS).

Presented by
Presented by Dr. Aled Jones, Senior Product & Applications Manager, ProZyme (a part of Agilent).

Topics Covered

  • Choosing a glycan derivatization scheme
  • LC/FLD and LC/MS analysis of released N-glycans
  • Advice on analytical detection method and fluorescent dye choice

Presenter Information »

 
 

N-Glycan Sample Preparation and Analysis Workflows for Screening and Characterization of Biotherapeutics

This month’s webcast focused on workflows for the analysis of N-glycans.  The CHROMacademy material provided in this month’s tutorial will provide you with even more information on how glycosylation affects biotherapeutics, the importance of characterizing this post translational modification, and the techniques used to analyze N-glycans.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will provide fundamental knowledge and practical information relating to N-glycan analysis.
 
eLearning Modules

Reversed Phase – Introduction and Peptide Level Analysis

This module provides an introduction to the use of reversed phase HPLC for the analysis of biopharmaceuticals at the peptide level; detailing the information which can be gained..

Analysis at the Peptide Level | mAb Peptide Map
mAb Post-Translational Modifications | Glycoprofile

 

HILIC

This module comprehensively covers the use of HILIC for the analysis of biopharmaceutical, in particular its applicability to glycan analysis.

Introduction to HILIC for Biopharmaceuticals | Glycan Mapping Workflow
Typical Glycan Mapping HILIC Conditions | Glycan Analysis During Biosimilar Development

 

Quick Guides


HILIC, IEX, and SEC for the Analysis of Biomolecules

Biomolecules present unique challenges to analytical scientists in comparison to their small molecule counterparts.  Learn how to apply HILIC, IEX, and SEC to the analysis of complex biomolecules, which parameters should be considered, and the common pitfalls which may occur.

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 

Biopharmaceuticals Quiz

Test your knowledge of biopharmaceuticals and their analysis with this quick quiz.

Take the Biochromatography Quiz

 
 

Techniques Employed in Biopharmaceutical Analysis – Part I Reversed Phase and HILIC

Biopharmaceuticals offer great new possibilities and potential in treating various life-threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required for their characterization and analysis. Reversed phase chromatography, the mainstay of traditional small molecule analysis, is employed at multiple biopharmaceutical levels due to its versatility, inherent relative efficiency and ease of hyphenation to a mass spectrometer. However, these separations often rely on finely balanced critical parameters being correctly optimized. We shall interrogate these critical parameters at each level and discuss best practices for implementation and optimization. The considerable increase in glycosylated biopharmaceuticals and the development of biosimilars and biobetters has led to much greater interest in the type and degree of glycosylation. We will review not only the popularity behind glycosylated biopharmaceuticals but where the heterogeneity comes from and how glycans can be effectively liberated, characterized and analyzed using HILIC.

Techniques Employed in Biopharmaceutical Analysis – Part I Reversed Phase and HILIC »

 

Choosing the Correct Column and Mobile Phase for LC-MS Characterization of Biologics

This webcast will focus on the parameters which are essential for the development of a successful RPLC-MS method for the characterization of a monoclonal antibody. The methods will allow for the analysis of the intact species, determination of absolute mass, examination of the major fragments, (Fab/Fc and Hc/Lc), and investigation of the small peptides produced by protease digestion.  The importance of pore size and mobile phase additives will be addressed and guidance given on how to optimize methods for sensitivity and resolution.

Choosing the Correct Column and Mobile Phase for LC-MS Characterization of Biologics »

 

Choosing the Correct Column and Mobile Phase for LC-MS Peptide Mapping

This webcast will focus on essential parameters for successful peptide mapping by LC/MS. Obtaining complete sequence coverage as well as separation and detection of low-level post-translational modifications is a challenging endeavor. Guidance will be given on matching column, mobile phase, and detector for optimum sequence coverage. Additionally, optimization of key method conditions such as gradient length and temperature will be discussed.

Choosing the Correct Column and Mobile Phase for LC-MS Peptide Mapping »

 
 
 
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Dr. Aled Jones,
Senior Product & Applications Manager,
ProZyme (a part of Agilent).


Aled is a Senior Product & Applications Manager at ProZyme (a part of Agilent Technologies), focusing on tools for glycobiology. 

He received his B.Sc. and Ph.D. degrees in biochemistry from Cardiff University, completing graduate work in the connective tissue biology laboratories. 

This was followed by a postdoctoral fellowship at Wyeth Research in Cambridge MA, where he subsequently worked as a Senior Research Scientist.