Chromatography for Food and Beverage

The CHROMacademy reversed phase chromatography module is a comprehensive guide to this ubiquitous technique.

The aim of this module is to introduce some of the working principles of ion chromatography and related techniques.

The aim of this module is to give an overview of the mechanism of normal phase chromatography (NP-HPLC) and explain the basis of the retention mechanism.

The aim of this unit is to introduce hydrophilic interaction liquid chromatography (HILIC) as a novel mode of chromatography. We will present both advantages and limitations of the HILIC separation mode.

This module focuses on size exclusion chromatography for the analysis of biomolecules.

The aim of this module is to explain the problems that can be encountered when using isocratic HPLC, and demonstrate how these problems can be overcome using gradient HPLC. 

Join us for a little over an hour on this step-by-step guide through setting up an HPLC instrument. 

This course considers the wide array of choices available to method developers and teaches a refined and logical approach to method development.

This webcast will allow you to understand how to select a stationary phase based on analyte chemistry, how particle morphology can be used to improve resolution, and how column dimensions can be altered to increase throughput.

In this module, the basic principles of liquid/liquid extraction (LLE) are described, as well as an overview of limitations and drawbacks of LLE protocols. Specific advice is given for various problems that are routinely encountered. 

This module introduces the concept of functional group chemistry as a means of targeting separation mechanisms in liquid and solid phase extraction (SPE). It explains the different types of functional group chemistry and investigates various physiochemical principles associated with different functional groups.

This module will describe the underlying scientific principles behind QuEChERS allowing you to fully understand and successfully apply the process. 

This quick guide details how Soxhlet extraction works, parameters that require optimization, and how improvements to the methodology and automation have allowed an increase in throughput and improved reproducibility. 

This module fully investigates the various aspects of practical SPE, as well as describing the basic principles and stages of method development in SPE.

The primary aims of any sample preparation technique are to provide clean samples and enrich analytes to reduce limits of detection (LOD). 

This quick guide will provides hints and tips for optimizing filtration using syringe end filters.

In this module we will follow two very experienced analysts as they guide you through the process of managing your workspace an samples in a way that eliminates errors.

The focus of this module is to provide you with practical information to improve your pipetting technique - this encompasses the use of pipettes, how to select the correct pipette and tip, how to and why we calibrate pipettes, as well as how to maintain them in order to have confidence that they are functioning reliably.

This bootcamp will empower you to correctly operate your instrument ensuring longer up-time, less troubleshooting, and more robust data acquisition.

This module will detail the process of obtaining and extracting spectra for library matching, what is involved in the matching process, and interpreting match results. As well as providing learning on troubleshooting poor library matching.

For GC, switching an existing method from helium to hydrogen can often be relatively straightforward. For GC-MS though, there can be major challenges in making the switch. This module explores the common challenges and strategies that can be employed to resolve these.

The basic principles of mass analysis using time-of-flight mass analyzers are relatively straightforward in comparison to many of the other typical mass analyzing devices.

In quadrupole mass analyzing devices, electric fields are used to separate ions according to their mass-to-charge ratio (m/z) as they pass along the central axis of four parallel equidistant rods (or poles) that have fixed (DC) and alternating (RF) voltages applied to them. 

Contamination is usually identified by excessive background ions in the mass spectrum. Contamination can originate from GC or MS components in the system.

This quick guide will detail how to determine the solvent delay time for your method and outline the benefits of using this in every method.

A GC-MS tune report is invaluable as it provides information on how well the instrument is working, indicates if cleaning or maintenance is required, serves as a chronicle of how the instrument has performed in the past, and can be used to diagnose problems when troubleshooting.

With a strong emphasis on practical technique, this course is for HPLC operators who need to perform error-free routine analysis to a high level of consistency, accuracy and efficiency.

With a strong emphasis on practical technique, this course is for GC operators who need to perform error-free routine analysis to a high level of consistency, accuracy and efficiency.

With a strong emphasis on practical technique, this course is for GC-MS operators who need to perform error-free routine analysis to a high level of consistency, accuracy and efficiency.

A learning path on HPLC system suitability covers method validation, key chromatographic parameters (precision, accuracy, resolution, tailing factor, and theoretical plates), and calculation methods.

This learning path on quantitation, calibration, and integration in HPLC covers external and internal standard methods, calibration curves, and linearity assessment. 

A learning path on GC without helium carrier gas covers alternative gases like hydrogen and nitrogen, their impact on efficiency, resolution, and detector compatibility. 

This learning path on GC column fundamentals covers stationary phase chemistry, column dimensions (length, diameter, film thickness), and phase selection for different analytes. 

This learning path on troubleshooting GC-MS covers sensitivity issues, interpreting tune reports for ion optics, mass accuracy, and detector performance.

This learning path on troubleshooting LC-MS covers common issues like low sensitivity, ion suppression, peak tailing, and mass accuracy errors. 

This comprehensive training course has been designed to give new analysts all the skills they need from essential wet chemistry techniques, through to instrument setup, in-depth knowledge of the instrument components, and fundamental understanding of the scientific theory of separation science.