CHROMacademy Classroom

HPLC Method Development

Live Online on 15th - 16th September

Instructor-led Training

This instructor-led HPLC Method Development training course provides a comprehensive, process-driven approach to developing optimal and robust High-Performance Liquid Chromatography (HPLC) methods for analytical laboratories.

Designed for experienced chromatographers, the course delivers a detailed exploration of the critical parameters involved in HPLC method development and demonstrates how these are combined into a practical, step-by-step development process. Participants will learn how to apply chromatographic theory to support a structured and efficient approach to developing commercial-quality HPLC methods.

The course examines each stage of the HPLC method development workflow, including the selection and optimization of key chromatographic parameters, while focusing on practical strategies that improve method performance, reproducibility, and robustness.

A strong emphasis is placed on Quality by Design (QbD) principles, helping participants understand how robustness can be built into each stage of development while avoiding common chromatographic issues and method weaknesses.

This advanced HPLC course is ideal for experienced chromatographers, analytical chemists, and laboratory scientists involved in HPLC method development, optimization, and analytical problem solving.

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CHROMacademy Classroom

Delivery Details

Duration: This course is delivered over two Live 4 hour sessions. Run on consecutive days.
                      Each daily session runs from 9am – 1pm (Eastern Time Zone).

Price: $695 

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Course Overview

What You Will Learn: 

  • Setting method development objectives
  • Sample preparation
  • System choices
  • Choosing a column and mobile phases
  • Optimizing strategies
  • Quantitation and system characterization 

Previous Experience: 

Delegates should have a good knowledge of chromatography and experience as HPLC users. Some experience in method development is recommended. A good grounding in chemistry is also beneficial.

Course content: 

Introduction

  • Design specification
  • What is QBD?
  • Building in robustness

Influencing Resolution

  • Retention factor
  • Efficiency
  • Selectivity

Sample Preparation

  • Influencing sample solubility
  • Choosing a diluent

Mobile Phase

  • Sample polarity
  • Organic modifiers
  • pH control
  • Flow rates

Columns

  • Physical dimensions
  • Particle type, sizes, and UHPLC
  • Temperature
  • Chemically modified silica
  • Reversed phase ligands
  • Hydrophobic subtraction model classification

Gradient Elution

  • Isocratic or gradient
  • Optimizing k’
  • Effect of each gradient parameter

Building a Practical Process

  • Sample preparation
  • Retention
  • Screening for alpha
  • Chromatographic optimization
  • Linearity, range and sensitivity
  • Pre-validation

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