CHROMacademy Classroom

Understanding HPLC

Live Online on 25th - 26th August

Instructor-led Training

This course introduces the essential concepts of High-Performance Liquid Chromatography (HPLC) analysis, providing participants with a broader understanding of HPLC theory, instrumentation, and practical laboratory applications.

Topics covered include HPLC hardware fundamentals, modes of analysis, column chemistry, troubleshooting strategies, and critical mobile phase parameters such as solvent type and proportion, pH, buffer strength, and related method variables that influence chromatographic performance.

Designed for participants with some hands-on HPLC experience, the course develops a deeper understanding of the complete HPLC process while focusing on practical, day-to-day laboratory applications. Participants will build confidence in HPLC operation, improve analytical efficiency, and reduce common errors to become more self-sufficient in routine HPLC practice.

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CHROMacademy Classroom

Delivery Details

Duration: This course is delivered over two Live 4 hour sessions. Run on consecutive days.
                      Each daily session runs from 9am – 1pm (Eastern Time Zone).

Price: $695 

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Course Overview

What You Will Learn: 

  • Principles of chromatography and how this relates to HPLC
  • Understanding of the chromatographic parameters
  • Hardware overview – the function of each HPLC module
  • The essential principles of mobile phases, buffers and eluent additives for HPLC
  • HPLC column parameters and common stationary phases 

Previous Experience: 

Previous experience is not essential however delegates would benefit from a basic understanding of HPLC and some previous practical experience of HPLC.

Course content: 

The Chromatographic Process

  • Overview of the reversed phase HPLC process
  • Revision of polarity, solubility, and diluents
  • Introduction to chromatographic parameters: capacity factor, selectivity, efficiency, and resolution

HPLC Mobile Phases and Additives

  • Mobile phase preparation
  • Organic modifiers
  • Acids, bases pH control, and buffering
  • Ion pairing agents

HPLC Columns

  • Column dimensions
  • Silica based supports
  • Bonded phases
  • The van Deemter Equation
  • Common reversed phase column chemistries

Introduction to Instrumentation

  • The pump
  • The autosampler
  • Detectors - UV, RID, F, ELSD, CAD, ECD

Introduction to Other Modes of HPLC

  • Reversed phase
  • Ion suppression and ion pair in RP
  • Normal phase
  • HILIC
  • Size exclusion
  • Ion exchange

Data processing

  • Peak integration
  • Standards and calibration
  • System suitability

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