Chromatography Training for Pharmaceutical Analysis

At the end of this module you will be able to estimate resolution between peaks within a chromatogram, explain how resolution is influenced by retention factor, selectivity, and efficiency, recognize which of these factors should be improved to increase resolution within specific separations, develop strategies to efficiently optimize resolution, and troubleshooting of separations in a practical context.

The aims of this module include the comparison and contrast of packed and capillary columns. A revision session of of fundamental intermolecular interactions is included in order to relate the various types of interaction to retention in GC.

Over four short videos we will look at how to correctly setup a GC autosampler. We will consider parameters that can be changed by the user, including the syringe and needle, needle wash routines, vials, injection speed, viscosity delay, and dwell time.

This module outlines some of the common system suitability tests we see in GC analyses.

This module will look at the reasons why we would need to use hydrogen generators, how hydrogen is generated, and how to select the correct generator size. As well as discussing some of the common maintenance points for these pieces of instrumentation.

A step by step guide to setting up a GC instrument. From checking the cylinders, dismantling the inlet, to correctly installing a column and lighting an FID. 

The aim of this module is to highlight the various detector types available for GC and describe the performance characteristics associated with these detectors.

The aims of this module include illustrating the relationship between temperature and retention in GC, and highlighting the advantages and disadvantages of isothermal and temperature programmed GC separations.

This course has been designed for those who are experience in GC and who are now looking to develop their experience and understanding of method development.

This module takes an initial look at the science behind the most commonly used sample preparation techniques.

This module introduces the terminology associated with solid phase extraction (SPE). It explains the chemistry and physical properties associated with SPE sorbents and substrates. 

The purpose of this module is to highlight the various steps in the protocol for various modes (sorbents) for conventional solid phase extraction procedures.

This module fully investigates the various aspects of practical SPE, as well as describing the basic principles and stages of method development in SPE.

This module introduces the concept of functional group chemistry as a means of targeting separation mechanisms in liquid and solid phase extraction (SPE). 

The focus of this module is to provide you with practical information to improve your pipetting technique.

The simple task of processing the SPE columns or plates is not free of error, therefore, this article looks at the methods used for SPE processing and decides which is best.

The primary aims of any sample preparation technique are to provide clean samples and enrich analytes to reduce limits of detection (LOD). The sample preparation technique should ideally be cheap, easy, fast, and compatible with a range of analytical instrumentation.

Derivatization will inherently add time, complexity, and cost to the analytical workflow, however, this quick guide will help to determine if derivatization is suitable for your application by considering reasons for implementing derivatization and how this can be done.

This module will explore the instrument acquisition methods used and examine the type of data that can be produced from such systems.

This module will detail the process of obtaining and extracting spectra for library matching, what is involved in the matching process, and interpreting match results. As well as providing learning on troubleshooting poor library matching.

This module will explain the differences between the data generated, provide you with details on the parameters which can be set to acquire the data, and the impact of the acquisition mode and parameters on the sensitivity of that data.

In this webcast we explore the principles of fragmentation produced by electron impact ionization in GC-MS. 

Library matching of extracted mass spectra is key to confirming peak identity in GC-MS. This module covers how spectra are obtained, matched, and interpreted, along with tips for troubleshooting poor matches.

Time-of-flight mass analyzers work by extracting ions in short bursts, accelerating them, and measuring their flight down a fixed-length evacuated tube. Their principles are simpler than many other analyzers.

This quick guide will detail how to determine the solvent delay time for your method and outline the benefits of using this in every method.

In this webcast, we explore the theory, technology, and applications of GC-MS/MS. We will discuss the key features of GC-MS/MS instrumentation alongside an overview of the key instrument operating principles and theories.

With a strong emphasis on practical technique, this course is for HPLC operators who need to perform error-free routine analysis to a high level of consistency, accuracy and efficiency.

This learning path on quantitation, calibration, and integration in HPLC covers external and internal standard methods, calibration curves, and linearity assessment. 

This learning path on HPLC method reliability covers robustness, precision, accuracy, and reproducibility. It includes system suitability testing, column stability, mobile phase consistency, and instrument performance monitoring. 

With a strong emphasis on practical technique, this course is for GC operators who need to perform error-free routine analysis to a high level of consistency, accuracy and efficiency.

With a strong emphasis on practical technique, this course is for GC-MS operators who need to perform error-free routine analysis to a high level of consistency, accuracy and efficiency.